Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Regulation of MicroRNA-378 expression in mature human adipose tissue cells by adiponectin, free fatty acids and dexamethasone

Lulian Xu, Li Jiang, Kefeng Gu, Zhenzhen Liu, Xu Xu

Department of Pediatric Endocrinology, Wuxi Childrenâ€™s Hospital, No. 299 Qingyang Street, Wuxi, China;

For correspondence:- Xu Xu Email: jg9048@163.com

Accepted: 20 October 2017 Published: 31 January 2018

Citation: Xu L, Jiang L, Gu K, Liu Z, Xu X. Regulation of MicroRNA-378 expression in mature human adipose tissue cells by adiponectin, free fatty acids and dexamethasone. Trop J Pharm Res 2018; 17(1):29-34 doi: 10.4314/tjpr.v17i1.5

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effects of adiponectin (ADPN), free fatty acids (FFAs), growth hormone (GH), and dexamethasone (DEX) on miR-378 expression in human adipose tissue cells, and their influence on regulation of obesity and insensitivity to insulin.

Methods: Human pre-adipocytes were cultured and differentiated. Adipocytes were treated with ADPN, FFAs, GH and DEX. RNA was isolated and quantified by real-time polymerase chain reaction (RT-PCR).

Results: Stimulation with FFA led to significant up-regulation of the expression of MiR-378 (approximately 3.8-fold) relative to control at the 4th hour (p < 0.01) in human mature adipose tissue cells. The expression of MiR-378 was increased almost 1.5-fold by ADPN within 24 h, relative to untreated control (p < 0.05).

Conclusion: The results of this study demonstrate that miR-378 expression is influenced by FFAs, ADPN, and DEX, the interaction of which may be involved in the pathogenesis of obesity-induced insensitivity to insulin. Thus, miR-378 is a potential biomarker for predicting the risk of complications, especially insulin resistance in obesity

Keywords: MiR-378, Adipocytes, Adiponectin, Free fatty acids, Growth hormone, Dexamethasone, Obesity, Insulin resistance